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# IncP-1beta multiresistance plasmid pB8

>pB8_006 hypothetical protein
MSKQAVFTMKLEPELRAEFMAEAEAAHRPASQVLRELMREFVQRQRESRE
YDEFLRRKVEAGRASMRAGLGRSNDEVEAEFAARRASVASQA
>pB8_001 'merR, MerC protein (truncated)
MREKMADLARMEAVLSELVCACHARRGNVSCPLIASLQGGASLAGSAMP
>pB8_051 kfrA, KfrA protein
MAISKEQIFAVADELDAAGQNPTLANVRKQLGSGSFTTISEAMNEWRARK
ASQAAPIREPAPQAITDKLAELGGDLWAVALEMANNRLAAEREALEAVRQ
ETEAARQEAAELADQLTGELDEAKARVAALEAVEAAAKGEADELRGKLAA
TSERAATAEARAGELRTELDHAHQEARQARAERDKAQERATASADQVEAL
RADLAAANSRTAEIERRAGELRADLERANQATDQARAAQAEQRKATEVAA
AERDQVRAELVKVQAKAEAAEQAHQEQRKAMAAEAHRQAERLTAAQGERD
QAKRDAAQAREEAAGLRGRLEALETVMAQGRAADPTSGGRKKT
>pB8_050 kfrB, KfrB protein
MKERLLVMNGQRIVQAEKDGAWTNQKVDKAGALKPGIYNLYTAQAADKKQ
THAGVIVHADATNVYQQIGKNFVMHARSDFDKVPEIGSAKSISYNAQGKA
AVAAEAPKLTRGRSM
>pB8_049 kfrC, KfrC protein
MKRLTIPGSGTESRATKPARVSAPATLGGAAFGASREDTGADLLEAAQAA
EIEQQATLEAAPVEQSYPETLALYVQAKHDQVEHIEDRLENLIDRQQARL
QQTQASAPGRLSLPGSKRAWQNQQAQQQARLQTLHARLEAVREIKEGMGL
HSPKIEELATRKMRAENPELASDWDAMREAARRHELLMRKQEQERKQSQA
QERPGRSQSLGLSARPA
>pB8_062 klcA, KlcA protein
MNTQDQPVTASLVAEAQRLDFLPTYFGPRLMMRGEALVYAWLRRLCERYN
GAYWHYYTLSDGGFYLAPDLAERLEIEVDGNGFRGELSADAAGIVATLFA
LGQLAAEIAGTDAADALIDRYHFLRGFAAGHPEAAAIYRAID
>pB8_061 klcB, KlcB protein
MAKRTKQPAQTGQDWSAPAAELLAELPADRDGLLAAAVAAVVEIDAAVMR
GDGAAAELAGDRYEAIIWKLNGGTNFGCMADDEAAGRVIERHCAAVPGDV
PLWGQRGQFLAVAGDVRALVEYEAGYGGPLNAHFQFHAVDLDRPFISATG
YRSHFDTARGCMTVDEVARGILTAMLAEKKRPVLIEANYRDRLADAPLPD
WLAGLVPPARREPATVTIPPGFVLVDVVLPAHKAFIARRWAAEAAGKVKA
ARAARSNAKGKAGGRERDPASAETAMRCSTAKADDCKAEAGPVSPEATMP
GAGEASCSTARNGDAGPADLVEFTPAPGQRCEIVSVHHPVFAKEIGKRVI
IVKVHPDTRQVWAHDDRPVTYKTNRAGRRVVDSDPSCIQSIYGFDQLRLI
T
>pB8_059 kleA, KleA protein
MSKNKIMPWVDALPNVEATDFQARRDQIEATMAEAAELVKQAEELRGKAY
FAALSLEASAKGEWSSQAVEQAKRSVGW
>pB8_058 kleB, KleB protein
MSKGKIEIIETCCRRCGKSIRTLSHTIIGADDAREKFGSICGGCITPEED
NELTEMLLAAAVRRMSGATLQ
>pB8_057 kleE, KleE protein
MSNIVKFPRASKPPAPEPVQPAAPAAAPAAPKAEGRGLVAGLVKFVWVAT
VLVWPVLKWVLAIITFFQFVRMLYHWNTPGVYAGWSFLAYFAALTAITYF
VSIYKPKGL
>pB8_056 kleF, KleF protein
MAKQTLPYPPGFVEPTTGRVAVMVREYADSDLNGDAPAYWYSAQSEEWGL
DPWRLVEGVDPHVGGGSFDVCFASGGTRTVGPLMTFFLSAAHAAQLIDAK
GEELALQRATLAVIADGLGLPAKALRIEAKVEGRPAVFYDQDGATLCACA
VDSDHWRQARATAATASAIDKARTNF
>pB8_055 kleG, KleG protein
MRHSSLTPRGKSWPCSAPPWPSSRTGWACLPRRCASRRRWRAGRPCSTTR
TGPRFARARWIPTTGDRRGRRPQRHRPSTRREQIFSR
>pB8_063 kluA, KluA regulatory protein
MRTTQQLSITLPNDMADVVKTKVKTGEYASESEVIRDGLRALLARDRAVE
SWLHNQVGPAYDALKADPSRAVTADQVRARLAAEHAKAQ
>pB8_064 kluB, plasmid stability protein
MSYAVVFSPEAEEQLAELYRYIAAAASPDVAARYTEAIVTYCESLRTFPL
RGTMRDDVRPGLRITNYRKRAVIAFDVAGDLVSIIGVFYGGQDYETILHD
DADD
>pB8_054 korA, KorA protein
MKKRLTEAQFQTAIKGLEIGQQTIDIARGVLVDGRPQAEFVTSLGLTKGA
VSQAVSRVWAAAGEQLPEGFERVTAVLPEHQAFIVKKWEADAKRKQEPKS
>pB8_060 korC, KorC protein
MTNDANIRLECLKPAERWAQPSGEEVREVLRLAGLTGGKAAKVLGLGPKG
DRTIRRWVGEDTPIPYAAWALLCDYAGLGLIWKEV
>pB8_004 tnpA, Transposase
MTTKSERLTVLSDAEQEALYGLPDFDDAQRLEYLALTETELALASSRPGL
HAQVYCILQIGYFKAKHAFFRFDWSEVEHDCAFVLSRYFHGESFEHKPIS
KHEHYTQREWIADLFGYRPWAAEFLAQLAQQAAQTVRRDVMPGFIAAELI
VWLNEHKIIRPGYTTLQELVSEALSAERRRLAGLLSEVLDESAKAALGRL
LVRDDTLSQLAALKQDAKDFGWRQMAREREKRATLEPLHRIAKALLPKLG
VSQQNLLYYASLANFYTVHDLRNLKADQTYLYLLCYAWVRYRQLSDNLVD
AMAYHMKQLEDESSAGAKQSFVAEQVRRQQDTPQVGRLLSLYIDDSVPDP
TPFGDVRQRAYKIMPRDTLQTTAQRMSVKPVSKLALHWQAVDGLAERIRR
HLRPLYVALDLAGTDPGSPWLVALAWAKDVFAKQQRLSQRPLAECPAATL
PKRLRPYLLTFDADGKPTGLHADRYEFWLYRQVRKRFQSGELYLDDSLQH
RHFSDELVSLDEKAAVLAQIDIPFLRQPLDAQLDALATELRAQWLAFNRE
LKQGKLTHLEYDKDTQKLTWRKPKGENQKAREKAFYEQLPFCDVADVFRF
VNGQCQFLSALTPLQPRYAKKVADADSLMAVIIAQAMNHGNQVMARTSDI
PYHVLESAYQQYLRHATLHAANDCISNAIAALPIFPYYSFDLDALYGAVD
GQKFGVERPTVKARHSRKYFGRGKGVVAYTLLCNHVPLNGYLIGAHDYEA
HHVFDIWYRNTSDIVPTAITGDMHSVNKANFAILHWFGLRFEPRFTDLGD
QLKELYSADDPALYDQCLIRPAGRIDRDLIVSEKPNLDQIVATLGLKEMT
QGTLIRKLCTYTAPNPTRRAVFEFDKLIRSIYTLRYLRDPQLERNVHRSQ
NRIESYHQLRSTIAQVGGKKELTGRTDIEIEISNQCARLIANAVIFYNSA
ILSRLLMKYEASGNAKAHALLTQISPAAWRHILLNGHYTFQSDGKMIDLD
ALVAGLELG
>pB8_039 traC2, TraC2 primase
MPITKAEAQGVTRAFVRDYPGALELAYKFREDAAELYGPRAAEVPADMKG
GYVPKETLHAGRAYRGRVDVPLQNVESASDLLMTLRHEVLGHYGANTFAP
GEKRALLDGLAAARNEPTLKPLWDDVNRRYAGQSLDVRAEEVFALHCEGI
EPSQHQVADQVQQRGQQSFTETCIARVRPMQADDLHNIVCMVAQGLRDRS
RTQQNFPQFNELFRRDENMEPKKPFHEVVAEKLIEQLKAGTAPWQKPWEP
GEPNAYLPMNPTTGKRYKGINAIHLMAQGRSDARWMTYKQAAAVGAQVRK
GEKGTPVQYWKFSEEQDKLDDSGRPVLDAKGQPVKETVMLERPRVFFATV
FNGEQIDGLPPLQPKKEQTWNAVERAEHILKASGATITHAAGDRAFYRPS
TDSITLPERGQFPSSDRYYATALHELGHWTGHASRLDRDLAHPFGSEGYA
KEELRAEIASMIVGDELGIGHDPGQHAAYVGSWIKALQDEPLEVFRAAAD
AEKIHDYVLAFEQKQVQEQDQQQSQAQDEAVAQALAVDIAEVLDNPDVSF
SHYQAFQGDTLEDALRSRGLETVGSITGTDPEQFYAVAHDRLSPVFGIDP
SHTDTDNAYLERKGLAQEFANMAEQLHLAQQLQQHGEQIVSSIDAEARWS
DGQRIFAFHDQDGEPHQVRSLDELNNYAPDQLMALPALTQQQAAVADQEA
NMTPPTIDQAAALLAAHPADAVQGIEQAAAARRQLAAGEIDGQAFADATR
QHLGVELPPDWSGELRIVGVAEQDGQTVDAAQAGIEPQAFQVYARKADAQ
FGEDAFAFVAGTRTEGQAEALAERLHLVDALGTDNQHERAAKLARVQEER
VRRDPNATEEDISAAKEARKTAEASAMLNDSDAQRRAAELERQERDRQQA
QPQAEKPERQYINVPYKEKDEAKSLGARWDRQQQSWYVPPGTDAAPFAKW
AQGAATAAVEPRSAQPAPEAQGEAQKPAQQAQQARQYLAVPYEQRNAAKA
AGALWDKAAKSWYVGPRADAAKLERWKPENVQAQQGPAMTPREEFAEAMR
SAGLFTGSNAQGDHPIMDGKRHRVPVEGGKKGALDGFYVGHLDGHPAGRI
INNKTGTDITWKSKGYALSDQEKAKLQAEAAEKLAQRAVEQDKAQEATAQ
RVGRQMADLVPIEQPTPYLQAKGIQAQAGVMTDREGQKTYIPAFDAEGKQ
WTMQYIQEDGTKRFAKDSKKEGCFHPVGGMDALAAAPALVISEGYATAAQ
VAEAVGHATVAAFDSGNLEAVAKALHAKFPDKPVIIAGDDDRHLVMTHGN
NPGREKAEAAAQAVGGKAIFPIFAPAENTYPRDLPAITPDSFKTHLRAEQ
RLADAAAGKVELAGDEAAKLKASMLSGAQIAALSTMKQHTDFNDLAHKSE
LGIEGVKRQIGAAISQVQRDEQQHQEQKHVEKKQQQIEQRPRRAARIG
>pB8_040 traD, TraD protein
MNEQTTTNTAAHDEPLAVLPPVDDDAAGREAVREKMADALTPGFQVEFDP
DEAERVGAFVEDALSEQDAAASGDDLVEVDGALEPAFLDDEGPSADIPPF
ITTTNARELYDLRPGETVAQAAARKASEG
>pB8_044 traH, TraH protein
MSEPKDQSIEDELDAALAALDSGPLPTSTLPEPQPSPEQATAGQPPAEAT
APTPAFTPPPSTGSPTLDALEENRRPKASTVCEHCPNSVWFASPAEVKCY
CRVMFLVTWSSKEPNQITHCDGEFLGQEQE
>pB8_044 traI, DNA relaxase
MIAKHVPMRSLGKSDFAGLANYITDAQSKDHRLGHVQATNCEAGSIQDAI
TEVLATQHTNTRAKGDKTYHLIVSFRAGEQPSADTLRAIEERICVGLGYG
EHQRISAVHNDTDNLHIHIAINKIHPTRHTMHEPYYPHRALAELCTALER
DYGLERDNHEPRKRGAEGRAADMERHAGVESLVGWIKRECLDEIKGAQSW
QELHQVMRDNGMELRVRANGLVFEAGDGTMVKASTVARDLSKPSLEARLG
PFEASPERQAQTTAKRQYRKDPIRLRVNTVELYAKYKAEQQSLTTARAQA
LERARHRKDRLIEAAKRSNRLRRATIKVVGEGRANKKLLYAQASKALRSE
IQAINKQFQQERTALYAEHSRRTWADWLKKEAQHGGADALAALRAREAAQ
GLKGNTIRGEGQAKPGHAPAVDNITKKGTIIFRAGMSAVRDDGDRLQVSR
EATREGLQEALRLAMQRYGNRITVNGTVEFKAQMIRAAVDSQLPITFTDP
ALESRRQALLNKENTHERTERPEHRGRTGRGAGGPGQRPAADQHATGAAA
VARAGDGRPAAGRGDRADAGLHAATVHRKPDVGRLGRKPPPQSQHRLRAL
SELGVVRIAGGSEVLLPRDVPRHVEQQGTQPDHALRRGISRPGTGVGQTP
PGVAAADKYIAEREAKRLKGFDIPKHSRYTAGDGALTFQGTRTIEGQALA
LLKRGDEVMVMPIDQATARRLTRIAVGDAVSITAKGSIKTSKGRSR
>pB8_045 traJ, oriT binding protein
MENDEKEHGRRRRQHLRVPVFPEEKDEIEANAKRAGVSVARYLRDVGQGY
QIKGVMDYQHVRELVRVNGDLGRLGGLLKLWLTDDVRTLQFGEATILALL
GRIEATQDEMSRIMKAVVQPRAEP
>pB8_046 traK, oriT binding protein
MPKTYPEELAEWVKGREAKKPRQDKHVVAFLAVKSDVQAALDAGYAMKTI
WEHMKETGRLRCRYETFTQHVKRYIKAAPVASPPPPATPPDSQPKGAKPE
PKAAPPASESKSEPPKIGGFTFDATPKKEDLL
>pB8_048 traM, TraM protein
MAADDKIEELIREIAAKHGIAVGRDDPILILQTINMKLMQDSASAQQEIL
DAFKSELESIAHRWGDDAKGKAERTLNAALAASKDAMTRGMQEGAKAAAE
AVRREVEAVTAQLVAPIREARRVAMMNMVAAGMAVVAAGLALWASL
>pB8_018 trbF, TrbF protein
MSIADTLKGLIFKKPANSAEPRDPRRSKEPLAGGRRAGEAENPYLAARRT
WNDHVGGVVSQRQTWQVIGILSLLIALAGVGGVIHIGSQSKFIPYVVEVD
KLGQTVAAGPVQAAGKADPRVIHAAVADWMSCARMVSPDVALQRKCVFKA
YSMLAPNDPATPKMNEWLNGTPDSSPFKRAEKEMVSVEIKTVIPQTPDTW
QVEWVETTRDRQGTLKGQPVTWRALVTTYIAEVTPNTTDEQLRNNPLSIY
VRDYSWSRIQ
>pB8_020 trbH, TrbH protein
MRKIVSLALLALALGLGGCATTSQYGNFVQSAALDQQKLATDAVQQLATL
YAPARTRLELQQPTPDPFGQALVKSLRDKGYALLEYAPAGASTQAPASAA
SQASAPATTAASGGLPLRYVLDQAGDSNLYRLTLMVGNQSITRPYLAQNG
TFAPAGYWVRKE
>pB8_023 trbK, TrbK protein
MKAIKALSLASAALVAALVAGCDNKPATAPMPEVNDENCKPENIAKIEDK
GVQQAFSSLCLRRGGDFKPSPKREW
>pB8_025 trbM, TrbM protein
MKKKLFALAALVAALGSTAGTASAQDVLTGDTRLACEAILCLSSGTRPSE
CTPSLSRYFNITKRKLSDTIRARLNFLQLCPVASQTPEMQSLVSAISRGA
GRCDAQSLNSTLVMWTGGYDDGRTYISNQLPDYCGAYTGHAYTDFASSGT
LPRYVGTPERGGYWVEARDYDRALAEYNERIRREDEERRRQSWLN
>pB8_027 trbO, TrbO protein
MDKFHAFMMRYTLGVGRLLQAYCKWAEGQAKNQLDLLLLGLGPIFALGLL
LWALPAWIGKPIAFVLSLPALYIIFLVLRAYAIRGGRR
>pB8_011 trfA, replication initiation protein
MQNEFNEQEYRRKLIEGGLSEADATDLAARTAAARRDSGSAGGAFRPAGS
LFELPATAGDNEAALPPKLPTPASAAVERSERMAQESAELARTMGLPPEP
RTAVNKMAEQIGALAQDKRSSAMATKKRTAGGELAEKVSEAKQTALLKHT
KQQIKDMQLSLFDLAPWPDHMRALPNDFGRSAIFTVRNKKVPRAALQGQS
IYHVNKDVEITYTGIELRADDDELVFAQVLEYAKRTALGEPVSFTFYELC
QDLDWSINGRYYTRAEECLTRLQASAMQFSSQRIGRLESVSLIRRFRVLD
RGKRTSRCQVEIDAEIVVLFAGDHYTKFVWEKYRKLSPTARRMFDYFATH
KEPYPLKLETFRLMCGSDSTRPKKWREQVGEACDELRENGLVESAWVNDD
LVHCKR